Abstract
In this paper, polyhexamethylene biguanidine hydrochloride (PHMB) oligomers were prepared by polycondensation of hexamethylenediamine, hydrochloric acid and 1,6-hexamethylenebis(dicyanamide); the organosilicon derivatives of PHMB oligomers (PHMB-Si) were prepared in solution through the reaction of PHMB and 3-Glycidoxypropyltrimethoxysilane (GLYMO). The analyses of FT-IR, 1H and 13C-NMR, elemental analysis, and TGA showed that the PHMB-Si was synthesized successfully. The relationship between antibiotic activity and viscosity-average molecular weight of PHMB; the relationship between antibiotic activity and the content of organosilicon in PHMB-Si were investigated. The experimental results revealed that the antibacterial activity of PHMB yielded the maximum at the viscosity-average molecular weight of 9000. The higher ratio of GLMYO in PHMB-Si, the higher antibiotic activity of PHMB-Si was. The minimal bactericidal concentration (MBC) of PHMB was 10 ppm, and the MBC of PHMB-Si was 5 ppm. The results showed there was an obvious synergy of PHMB-Si in bacteriostatic activity than PHMB.
Acknowledgments
The authors appreciate the help from Professor Z.Y. Qian and postgraduates S. Shi, Y. Qu, K. Shi for the experiment of cytotoxicity of PHMB and PHMB-Si on mice embryo fibroblasts 3T3 cells.