Immobilization of β-galactosidase on Polystyrene Microspheres Attached L-Alanine

Abstract

This study investigated the properties of immobilized β-galactosidase on polymeric beads having Schiff base. Polystyrene microspheres attached L-Alanine (FMPS-Ala) was synthesized from (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) and L-alanine by condensation. A coordinasyon polymer involving Ni²⁺(FMPS-Ala-Ni) was produced with the template method and characterized. β-galactosidase was immobilized onto the (FMPS), (FMPS-Ala) and (FMPS-Ala-Ni) complexes via covalent bonds. The Km/Vmax values were calculated as 0.343 mM/0.0259 mM min⁻¹for free β-galactosidase and 0.104 mM/0.0126 mM min⁻¹, 0.0617 mM/0.0417 mM min⁻¹and 0.210 mM/0.0287 mM min⁻¹for β-galactosidase immobilized to the (FMPS), (FMPS-Ala) and (FMPS-Ala-Ni) supports, respectively. The storage stability of (FMPS-Ala-Ni) was determined to be higher than that of the (FMPS) and (FMPS-Ala) polymers.

Keywords:

• Polymeric beads
• coordination polymer
• immobilization
• β-galactosidase
• poly(styrene)

Acknowledgments

This work was supported by Gazi University Research Fund (Project numbers: 05/2009-25 and 05/2012-68) for financial support.