Promising Macromolecular Conjugates of Camptothecin - the Synthesis, Characterization and in vitro Studies

Abstract

New macromolecular conjugates of camptothecin were prepared. The polymeric matrixes were obtained by the ring-opening polymerization of ϵ-caprolactone, glycolide, rac-lactide or trimethylene carbonate. The synthesized polymers and camptothecin were coupled via 1,6-diisocyanatohexane. The received macromolecular conjugates of camptothecin were characterized by ¹H and ¹³C-NMR or FT-IR spectroscopy. Toxicity of the received polymeric products were evaluated with bacterial luminescence test and two protozoan assays. Furthermore, the in vitro release of camptothecin from the obtained conjugates was investigated.

Keywords:

• Camptothecin
• macromolecular conjugates
• in vitro release
• biodegradable polymers
• bioresorbable polymers

Acknowledgments

The authors are indebted to Andrzej Jaklewicz from the Medical University of Warsaw for the AAS analysis.