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Journal of Macromolecular Science, Part A
Pure and Applied Chemistry
Volume 51, 2014 - Issue 9
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Original Articles

Isolation and Immobilization of His-Tagged Alcohol Dehydrogenase on Magnetic Nanoparticles in One Step: Application as Biosensor Platform

Ozge Kozgus GulduEge University, Institute of Nuclear Science, 35100Bornova-Izmir, Turkey
,
Selin EceEge University, Faculty of Science, Biochemistry Department, 35100Bornova-Izmir, Turkey
,
Serap EvranEge University, Faculty of Science, Biochemistry Department, 35100Bornova-Izmir, Turkey
,
E. Ilker MedineEge University, Institute of Nuclear Science, 35100Bornova-Izmir, Turkey
,
Dilek Odaci DemirkolEge University, Faculty of Science, Biochemistry Department, 35100Bornova-Izmir, TurkeyCorrespondence[email protected] [email protected]
,
Perihan UnakEge University, Institute of Nuclear Science, 35100Bornova-Izmir, Turkey
&
Suna TimurEge University, Faculty of Science, Biochemistry Department, 35100Bornova-Izmir, TurkeyCorrespondence[email protected] [email protected]
 show all
Pages 699-705 | Received 01 Mar 2014, Accepted 01 Apr 2014, Published online: 10 Jul 2014
 
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Abstract

His-tagged Alcohol dehydrogenase was produced as a recombinant protein in E. coli. Afterwards, isolation and immobilization of the enzyme was carried in one-step via copper modified magnetic nanoparticles (MNPs) by the effect of interactions between Cu and histidine. The resulting enzyme bound MNPs was then attached to the surface of carbon paste electrode by the magnetic force and used as an electrochemical biosensor for the alcohol sensing applications.

Funding

The authors thank the Ege University Research Funds (Project number: 2010-ILAM-003).

Supplemental Data

Supplemental data for this article can be accessed at www.tandfonline.com/lmsa.

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