An alternative approach to controlled release of oxprenolol from the implantable delivery system based on biodegradable copolymer and genistein

ABSTRACT

This paper describes the development of covalent star-shaped poly(L-lactide/ϵ-caprolactone) random copolymer-oxprenolol (OXP) conjugates as a potential approach to controlling drug release from implantable delivery systems. We prepared synthesized materials containing 14–17 mol% OXP, which were conjugated via an ester bond. The conjugates, which were composed of biodegradable copolyester chains, natural genistein as a central core and drug, were characterized by hydrogen nuclear magnetic resonance (¹H-NMR), Fourier transform infrared spectroscopy (FTIR) and viscosity methods. We evaluated the cyto- and genotoxicity of the synthesized copolymeric matrices, followed by the conjugates, with bacterial luminescence, protozoan and Salmonella typhimurium TA1535 assays. Furthermore, we performed in vitro mammalian assays of the obtained products with V79 cells. We found that the in vitro release of OXP from the obtained star-shaped conjugates was dependent on the structure of the synthesized biodegradable matrices.

KEYWORDS:

• Polymer-drug conjugates
• oxprenolol
• drug delivery system
• controlled release
• biodegradable polymers
• genistein

Acknowledgments

The authors are grateful to Dr Maria Wojewódzka from the Institute of Nuclear Chemistry & Technology, Center for Radiobiology & Biological Dosimetry in Warsaw for providing the Metafer Slide Scanning Platform.