Abstract
This paper reviews the versatile self-assembly of bidentate protein-binding agents on a duplex DNA scaffold, as demonstrated using the biotin-streptavidin system. The preferential binding and stabilization of bidentate biotin-containing duplexes is reflected in a 12°C increase in the melting temperature of the duplex in the presence of streptavidin.
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Acknowledgements
We thank the National Science Foundation and the National Institutes of Health (GM 35208) for financial support of this work.