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Original Articles

Photodimerisation of anthracenes using a DNA template and its analytical applications

, , , &
Pages 292-295 | Received 11 Jul 2008, Accepted 28 Sep 2008, Published online: 30 Apr 2009
 

Abstract

We tethered an anthracene group to one end of oligonucleotide to make anthracene–ODN conjugates (5AntODN15 and 3AntODN25). The sequences of the conjugates were designed to hybridise to adjacent sites of the template with their anthracene units stacked each other. The conjugates dimerised only in the presence of the template by light irradiation. The dimerisation efficiency was affected by one-base mismatch in the tandem duplexes. Furthermore, we studied the signal amplification experiments under thermal cycling in the presence of the targets with full match and mismatch sequences.

Acknowledgements

This work was partially supported by a grant-in-aid for JSPS fellow (18809000) (M.M) and for Scientific Research (B) (20350038) from JSPS (T.I.). The authors are also grateful to the support from PRESTO, JST (T.I.).

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