Calixarenes functionalised water-soluble iron oxide magnetite nanoparticles for enzyme immobilisation

ABSTRACT

In this study, we first used water-soluble iron oxide nanoparticles for Candida rugosa lipase immobilisation. Moreover, two new complexation phenomena of the prepared water-soluble Fe₃O₄ nanoparticles with an enzyme might address interesting results in terms of enzyme activity and stability in typical enzymatic reactions. The catalytic activity on pH and temperature dependence, and reusability of the immobilised lipases (p-SCX4-NP-E and p-SCX8-NP-E) were also investigated with the hydrolysis reaction of p-nitrophenyl palmitate (p-NPP). The results show that the highest catalytic affinities for p-SCX4-NP-E and p-SCX8-NP-E were obtained at pH 5.0 and pH 7.0, respectively.

Graphical abstract

KEYWORDS:

• Calix[n]arene
• water-soluble Fe₃O₄
• lipase
• immobilisation
• nanoparticles

Acknowledgments

We would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK-Grant Number 119Z131), and the Research Foundation of Selcuk University (SUBAP-Grant Number: 18201080) for financial support of this work.

Disclosure Statement

There are no conflicts to declare.

Additional information

Funding

The authors thank the Scientific and Technological Research Council of Turkey [119Z131] and the Scientific Research Projects Foundation of Selcuk University [18201080] for financial support of this work.