Magnetic poly ε-caprolactone nanoparticles containing Fe₃O₄ and gemcitabine enhance anti-tumor effect in pancreatic cancer xenograft mouse model

Abstract

We prepared magnetic (Fe₃O₄) poly ε-caprolactone (PCL) nanoparticles (mean diameter 164 ± 3 nm) containing an anticancer drug (gemcitabine) using emulsion-diffusion method in order to develop more efficient drug delivery for cancer treatment. Nanoparticles were smooth, well individualized and homogeneous in size. The values of magnetizations for the magnetic PCL nanoparticles were observed around 10.2 emu/g at 2000 Oe magnetic field intensity and showed super-paramagnetic property. In case of the drug, the drug loading contents was 18.6% and entrapment efficiency was 52.2%. The anti-tumor effects caused by these particles were examined using nude mice bearing subcutaneous human pancreatic adenocarcinoma cells (HPAC) in vivo. We divided that these mice were randomly assigned to one of five treatment groups for experimental contrast. The antitumor effect was showed with 15-fold higher dose when compared to free gemcitabine. From the result, the magnetic PCL nanoparticles may provide a therapeutic benefit by delivering drugs efficiently to magnetically targeted tumor tissues, thus achieving safe and successful anti-tumor effects with low toxicity.

Keywords: :

• Magnetic nanoparticles
• drug targeting
• pancreatic cancer
• gemcitabine
• in vivo

Abbreviations
PCL	=	poly ε-caprolactone
Gem	=	gemcitabine
PCL/Gem	=	PCL containing gemcitabine
PCL/Gem-magnet	=	PCL containing gemcitabine and magnetic field applied

Abbreviations
PCL	=	poly ε-caprolactone
Gem	=	gemcitabine
PCL/Gem	=	PCL containing gemcitabine
PCL/Gem-magnet	=	PCL containing gemcitabine and magnetic field applied