Erratum

This article refers to:

Below is the correct version of Table I and its corresponding legend that should have appeared in: Smith et al. Primary porcine brain microvascular endothelial cells: Biochemical and functional characterisation as a model for drug transport and targeting. J Drug Target 2007 May;15(4):253–268.

Table I.  TEER and permeability coefficients for sucrose and propranolol across PBMVEC monolayers grown alone, in the presence of C6 co-culture or exposed to C6 astroglial factors during growth. As a comparator permeability of b.End3, Caco-2 and the membrane alone is shown. PBMVECs maintain a significant transendothelial electrical resistance particularly when co‐cultured with C6 cells or when exposed to C6 astroglial factors and ‘switched’ to serum free hydrocortisone medium. The ratio of permeabilities (propranolol:sucrose) is indicative of the level of discrimination of the BBB model between transcellular and paracellular probes. PBMVECs unlike b.End3 discriminate between the paracellular and transcellular pathways.

	Permeability co-efficient ( × 10^− 6 cm s^− 1)		Ratio	TEER (Ω cm^2)
Blood–brain barrier model	[14C]Sucrose	[3H]Propranolol	(propranolol to sucrose)	Pre-switch	Post-switch
PBMVECs	12.1 ± 1.3	25.4 ± 1.8*	2.1 ± 1.4	44 ± 1.26	80 ± 13
PBMVECs exposed to C6 astroglial factors	8.8 ± 1.2	26.0 ± 1.3*	2.95 ± 1.1	173 ± 10	409 ± 35
PBMVECs with C6 co-culture	1.6 ± 1.0^†	18.4 ± 0.7*	11.5 ± 0.7	352 ± 34	834 ± 136
b.End3 with C6 co-culture	19.4 ± 2.6	23.4 ± 2.9	1.2 ± 1.1	66 ± 1.41	NA
Caco-2	1.6 ± 0.2^†	14.5 ± 0.9*	9.1 ± 0.2	512 ± 155	NA
Membrane (polyester, 4.7 cm^2)	49.3 ± 3.9	40.9 ± 5.1	0.8 ± 1.3	39.2 ± 0.9	NA

Sucrose and propranolol data quoted for PBMVECs is post-switch to serum free hydrocortisone medium. * Signifies statistical difference (P < 0.05, Student's t-test) between sucrose and propranolol permeability

^† Signifies statistical difference (P < 0.05, Student's t-test) between sucrose permeability compared to all other cells. Data are mean ± SD with n = 4 replicates.