Targeting of stabilized plasmid lipid particles to hepatocytes in vivo by means of coupled lactoferrin

Abstract

For non-viral gene delivery we prepared stabilized plasmid lipid particles (SPLPs), to which lactoferrin (LF) was coupled as a hepatocyte specific targeting ligand. LF–SPLPs and untargeted SPLPs labeled with [³H]cholesteryloleyl-ether were injected into rats. About 87% of the LF–SPLPs were eliminated from the blood within 5 min, while 80% of untargeted SPLPs were still circulating after 2 h. Fifty-two percent of the LF–SPLPs were taken up by hepatocytes, while non-parenchymal liver cells accounted for 16% of the uptake. Despite the efficient targeting of LF–SPLPs to hepatocytes and their capacity to transfect HepG2 and COS-7 cells in vitro, expression of a reporter gene was not detected in vivo. Overall, covalent coupling of LF to SPLPs leads to massive delivery in hepatocytes after systemic administration. However, these LF–SPLPs are not able to transfect these cells in vivo.

Keywords: :

• Stabilized plasmid lipid particles
• lactoferrin
• gene targeting
• hepatocytes
• non-parenchymal liver cells