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Abstract
Plasmid DNA was mixed with polyethyleneimine (PEI) and hyaluronic acid (HA) to afford ternary complexes with negative surface charge regardless of the mixing order. They showed reduced non-specific interactions with blood components. When DNA and PEI were mixed at a high concentration such as that used in in vivo experiments, they soon aggregated, and large particles were formed. On the other hand, pre-addition of HA to DNA prior to PEI effectively diminished the aggregation, and 10% (in volume) of the complexes remained as small particles with a diameter below 80 nm. Those negatively charged small ternary complexes induced a much stronger extra-gene expression in tumor than binary DNA/PEI complex after intratumoral or intravenous injection into the mice bearing B16 cells.
| Abbreviations | ||
| DAPI | = | 4′,6-diamidino-2-phenyl indole |
| DMSO | = | dimethyl sulfoxide |
| EMEM | = | Eagle's minimum essential medium |
| FITC | = | fluorescein isothiocyanate isomer-I |
| FBS | = | fetal bovine serum |
| GFP | = | green fluorescent protein |
| HA | = | hyaluronic acid |
| ME | = | mercaptoethanol |
| PEG-C | = | PEG derivative, having carboxylic acid side chain |
| PEI | = | polyethyleneimine |
| TR-HA | = | Texas Red-labeled HA |
| TBA-HA | = | HA tetrabutylammonium salt |
| Abbreviations | ||
| DAPI | = | 4′,6-diamidino-2-phenyl indole |
| DMSO | = | dimethyl sulfoxide |
| EMEM | = | Eagle's minimum essential medium |
| FITC | = | fluorescein isothiocyanate isomer-I |
| FBS | = | fetal bovine serum |
| GFP | = | green fluorescent protein |
| HA | = | hyaluronic acid |
| ME | = | mercaptoethanol |
| PEG-C | = | PEG derivative, having carboxylic acid side chain |
| PEI | = | polyethyleneimine |
| TR-HA | = | Texas Red-labeled HA |
| TBA-HA | = | HA tetrabutylammonium salt |