Titanium surface polyethylene glycol hydrogel and gentamicin-loaded cross-linked starch microspheres release system for anti-infective drugs

Abstract

Objective

To design and construct a hydrogel drug-controlled release system loaded with gentamicin on a titanium surface, and to evaluate the in vitro drug release behaviour and antibacterial properties and biocompatibility of the controlled release system.

Methods

Titanium (Ti) surface was coated with poly dopamine (PDA) substrate, and then polyethylene glycol (PEG) was attached to PDA. The composite drug microsphere controlled release layer formed by gentamicin (GEN) and cross-linked starch (CSt) were subsequently covered with poly lactic⁃co⁃glycolic acid (PLGA) as a barrier to construct a Ti-GEN-Cst-PLGA anti-infective drug controlled release system.

Results

The hydrogel drug release system was successfully constructed. The results of in vitro anti-staphylococcus aureus (SAU) assay, anti-staphylococcus epidermidis (SEP) assay and anti-Escherichia coli (ECO) assay showed that Ti-GEN-Cst-PLGA could effectively inhibit the growth of three bacteria. Assay in the New Zealand rabbit found that Ti-GEN-Cst-PLGA could promote wound healing at the 3rd week after implantation, and the pathology assay found that the Ti-GEN-Cst-PLGA group had less inflammatory reactions and significant tissue proliferation at the endophyte contact surface.

Conclusion

Ti-GEN-Cst-PLGA can effectively inhibit the inflammatory response and promote wound healing, or may be a potential treatment for orthopaedic endophytes.

Keywords:

• Titanium
• gentamicin
• hydrogel drug release system
• antibacterial

Authors’ contributions

Yunfeng Wu and Xuesong Zhang contributed to the conception and design of the study; Fanqi Hu contributed to the acquisition of data; Xiaoqing Yang and Shaofu Zhang performed the experiments; Chengqi Jia and Xiaole Liu contributed to the analysis of data; Yunfeng Wu wrote the manuscript; All authors reviewed and approved the final version of the manuscript.

Availability of data and material

The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.

Disclosure statement

The authors declare that they have no competing interests.

Ethics approval and consent to participate

This research was approved by the Ethics Committee of The Eighth Medical Centre, Chinese PLA General Hospital. All methods were carried out in accordance with relevant guidelines and regulations. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Funding

The author(s) reported there is no funding associated with the work featured in this article.