Abstract
Immunoglobulins (Igs) against anti-human white blood cells are putative contributors to the development of transfusion-related adverse effects, particularly transfusion-related acute lung injury (TRALI). Studies of Igs that are considered to be implicated in transfusion-related adverse effects have mainly focused on immunoglobulin G (IgG) class antibodies (Abs). In the authors' previous in vitro study, the association of polymorphonuclear neutrophils (PMNs) and lung microvascular endothelial (LME) cells was up-regulated in the presence of normal human serum–derived IgMs, when F(ab')2 fragments of IgMs were specific to low-affinity Fc receptors (FcR) for IgG, namely, Fcγ R III (CD16) and Fcγ RII (CD32). In this study, the authors found that CD7 antigen notably expresses in LME cells and that it acts as an Fc receptor for IgM in LME cells.
Notes
1Abbreviations. TRALI: transfusion-related acute lung injury; Ig: immunoglobulin; PMNs: polymorphonuclear neutrophils; LME cells: lung microvascular endothelial cells; Fc γ R: Fc receptor for IgG; Fc α R: Fc receptor for IgA; Fc μ R: Fc receptor for IgM; Fc ε R: Fc receptor for IgE; NK cells: natural killer cells; HLA: human leukocyte antigen; Ab: antibody; mAb: monoclonal antibody; pAb: polyclonal antibody; NHS: normal human serum; PE: phycoerythrin; FITC: fluorescein isothiocyanate; PBS: phosphate-buffered saline; FS: forward scatter; SS: side scatter; FCM: flow cytometry; BSA: bovine serum albumin; ADCC: antibody-dependent cell cytotoxicity.