Role of C825T Polymorphism of GNβ3 Gene in Preeclampsia

Abstract

Objective: The main objective of the study was to understand the role of C825T polymorphism that generates a splice variant in the β3 subunit of heterotrimeric G-protein in preeclampsia. Results: We analyzed genomic DNA of 151 women with preeclampsia (72 Caucasians and 79 African-Americans) and 198 women with normal delivery (102 Caucasians and 96 African-Americans) for C825T polymorphism of GNβ3 gene. The T-allele frequency in Caucasian women with preeclampsia was 0.42 as compared to 0.25 in normal pregnant women (p = 0.0004) and in African-American women with preeclampsia was 0.82 as compared to 0.68 in normal pregnant women (p = 0.0028). Conclusions: Results of these experiments show that the allele frequency of C825T polymorphism is significantly different in women with preeclampsia compared with women with normal delivery in Caucasian as well as African-American population.

Keywords:

• Preeclampsia
• Genomic analysis
• GNB3 protein
• Single nucleotide polymorphism
• SNP