Host Inflammatory Response Profiling in Preeclampsia Using an In Vitro Whole Blood Stimulation Model

Abstract

The pathophysiology of preeclampsia (PET) implicates an inflammatory dysfunction. This study profiled this host response by challenging whole blood with lipopolysaccharide. Multiplex immunoassays determined interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17, granulocyte/granulocyte macrophage-colony stimulating factors (G-CSF/GM-SCF), interferon(IFN)-γ, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein-1β and tumor necrosis factor (TNF)-α levels. Secretory capacity was expressed in pg/million white cells or monocytes (±SEM). PET featured significantly higher IL-1β, IL-2, IL-10, IL-13, G-CSF, IFN-γ, MCP-1 and TNF-α monocyte secretory capacities (p < 0.05). The PET group exhibited an inflammatory hyper-responsiveness (p < 0.01) which was poorly described by the traditional Th1:Th2 dichotomy.

Keywords:

• Cytokines
• Lipopolysaccharide
• In vitro
• Whole blood