Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis

ABSTRACT

Objective

Our study aimed to investigate IL9 and IL9R expression in preeclampsia and assess their effects on trophoblast biological behaviors.

Methods

IL9 and IL9R expression of placenta tissue were evaluated by immunochemistry and q-PCR. Using transwell, CCK-8, and tubule formation assays measured invasion, proliferation and angiogenesis of trophoblast with adding IL9 or anti-IL9R antibody.

Results

IL9 and IL9R levels were significantly decreased in preeclampsia. IL9 improved trophoblast activities. Blocking IL9/IL9R resulted in decreased proliferation, invasion, and tube-formation capability of trophoblast.

Conclusions

IL9 and IL9R contribute to the pathogenesis of preeclampsia. IL9/IL9R signaling provides a new potential therapeutic target for preventing preeclampsia.

KEYWORDS:

• IL9
• IL9 receptor
• trophoblast
• biological behaviors

Acknowledgments

The authors thank Pro Xiao-Tian Li and Hu Rong for providing excellent technical assistance and administrative assistance. This program was supported by the Shanghai Key Program of Clinical Science and Technology Innovation (17411950500; 18511105602), National Science Foundation of China (81971411; 81971408) and National Key R&D Program of China (2016YFC1000403).

Disclosure statement

The authors declare that there are no conflicts of interest.

Additional information

Funding

This program was supported by the Shanghai Key Program of Clinical Science and Technology Innovation [17411950500; 18511105602], National Science Foundation of China [81971411; 81971408] and National Key R&D Program of China [2016YFC1000403].