Assessment of Biodegradation of Oleandrin during Co-composting of Nerium oleander L. Wastes

ABSTRACT

Variations in the levels of the highly toxic oleandrin molecules were studied during composting of Nerium oleander L. waste mixed with clippings of the grass Pennisetum clandistenum L. The thermophilic phase is characterized by a rise in temperature, which reached 70°C. After 150 days of co-composting, the C/N ratio was 11, the pH was 8, the NO₃⁻/NH₄⁺ ratio was greater than 1 and overall decomposition reached 70%. During the successive stages of co-composting, oleandrin concentrations were monitored by HPLC. The relative abundance of oleadrin was 26.84% at T0 with 10% abatement during the first month and 90% after two months (stabilization phase), reaching 100%, i.e., total removal after 90 days of co-composting (maturation phase). The biodegradation of the toxic substance was largely attributed to the activity of actinomycetes and fungi. The germination index of lettuce and watercress seeds exceeded 50% after 90 days and reached 95% after 150 days, confirming that the final compost was mature, stable, and free from phytotoxicity in spite of the highly poisonous starting material.

ACKNOWLEDGMENT

The authors wish to thank Pr Moha Taourirt, Cadi Ayyad Unversity, Faculty of Sciences and Technic, for his helpful comments on oleadrin analysis.