ABSTRACT
The aim of this study was to synthesize chitosan nanoparticles loaded with rapeseed extract (RPE-CN) and to evaluate its therapeutic effects. The RPE-CNwas synthesized (Ion gelation), characterized (DLS, FTIR, atomic and electron microscopy) and their toxicity was evaluated by MTT method.Anti-inflammatory and pro-apoptotic effects were evaluated by qPCR method. Its antioxidant power was measured by ABTS, DPPH and FRAP methods and CAM method was used to evaluate its anti-angiogenic effects.Treatment with RPE-CN(Ps: 93.81 nm, PDI: 0.3, and ζ p: +35mv) reduced MCF7 cell viability and increased expression of genes associated with apoptosis (Caspase-3 and Caspase-9), inflammation (IL10) and antioxidant (SOD).RPE-CN exhibited notable inhibition in ABTS, DPPH, and Fe3+-TPTZ free radicals. Finally, angiogenesis in CAM tissue was suppressed by the down-regulation of VEGFR gene expression. According to the results, RPE-CN can be introduced as a potential chemo-preventive agent in the treatment of cancer.
Acknowledgments
The authors very much appreciate the support provided by the Islamic Azad University, Mashhad, Iran, throughout the present research.
Disclosure statement
No potential conflict of interest was reported by the author(s).