Biosynthesis of silver nanoparticles using Martynia annua and its antimicrobial and cytotoxic activities

ABSTRACT

The aim of present study, biosynthesis of silver nanoparticles (Ag NPs) was synthesized from silver ions using aqueous extract of the whole plant of Martynia annua and its Antimicrobial and Cytotoxic activities. The extraction is made by non-polar to polar solvent using Soxhlet apparatus. Initially, the aqueous (water) extract was analysed the primary phytochemical test and used for the synthesis of Ag NPs. The obtained Ag NPs were characterized using UV-vis. spectroscopy, XRD analysis, FTIR spectrum and HRTEM. The UV-vis spectroscopy surface Plasmon resonance centre at 443nm was confirmed the formation of Ag NPs. XRD analysis and SAED pattern was determining the crystalline nature of metallic silver. HRTEM image shows the morphology of Ag NPs contains spherical shapes with sizes 10-25nm. The extract and green synthesized Ag NPs have significant antimicrobial activity against both bacteria and fungi and also have good cytotoxicity against human lung cancer cell line A549  .

KEYWORDS:

• Martynia annua
• silver nanoparticles
• antimicrobial activity
• cytotoxicity activity

Acknowledgments

The authors gratefully acknowledge V.O. Chidambaram College, Tuticorin for FTIR analysis, Kalasalingam Academy of Research and Education, Krishnankoil, Virudhunagar for XRD analysis and thanks to PSG Institute of Advanced Studies, Coimbatore for HR-TEM analysis. Also, the authors thanks to Bharathidasan University, Tiruchirappalli, Smykon Biotech Pvt Ltd, Nagercoil, Pondicherry Centre for Biological Science and Educational Trust, Kottakuppam for biological experiments.

Disclosure statement

No potential conflict of interest was reported by the author(s).