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Abstract
We investigated hepatoprotective activity and antioxidant effect of the 2,5-dihydroxy-4,3′-di(β-d-glucopyranosyloxy)-trans-stilbene that purified from Morus bombycis Koidzumi roots against CCl4-induced liver damage in rats. The 2,5-dihydroxy-4,3′-di(β-d-glucopyranosyloxy)-trans-stilbene displayed dose-dependent superoxide radical scavenging activity (IC50 = 430.2 μg/ml), as assayed by the electron spin resonance (ESR) spin-trapping technique. The increase in aspartate aminotransferase (AST) activities in serum associated with carbon tetrachloride (CCl4)-induced liver injury was inhibited by 2,5-dihydroxy-4,3′-di(β-d-glucopyranosyloxy)-trans-stilbene and at a dose of 400 ∼ 600 mg/kg samples had hepatoprotective activity comparable to the standard agent, silymarin. The biochemical assays were confirmed by histological observations showing that the 2,5-dihydroxy-4,3′-di(β-d-glucopyranosyloxy)-trans-stilbene decreased cell ballooning in response to CCl4 treatment. These results demonstrate that the 2,5-dihydroxy-4,3′-di(β-d-glucopyranosyloxy)-trans-stilbene is a potent antioxidant with a liver protective action against CCl4-induced hepatotoxicity.
| Abbreviations | ||
| AST | = | aspartate aminotransferase |
| CCl4 | = | carbon tetrachloride |
| ROS | = | Reactive oxygen species |
| CCl3√ | = | trichloromethyl radical |
| ESR | = | electron spin resonance |
| RT | = | reverse transcription |
| DMPO | = | 5,5-dimethyl-1-pyrroline-oxide |
| TNF-α | = | tumor necrosis factor-α |
| MDA | = | malondialdehyde |
| 4-HNE | = | 4-hydroxynonenal |
| Abbreviations | ||
| AST | = | aspartate aminotransferase |
| CCl4 | = | carbon tetrachloride |
| ROS | = | Reactive oxygen species |
| CCl3√ | = | trichloromethyl radical |
| ESR | = | electron spin resonance |
| RT | = | reverse transcription |
| DMPO | = | 5,5-dimethyl-1-pyrroline-oxide |
| TNF-α | = | tumor necrosis factor-α |
| MDA | = | malondialdehyde |
| 4-HNE | = | 4-hydroxynonenal |