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Abstract
Nuclear factor-kappaB (NF-κB) has a dual role in the promotion or attenuation of cell death. Here, we demonstrated the role of NF-κB in the H2O2-induced death of astrocytes. H2O2 evoked the release of lactate dehydrogenase (LDH), a marker of cell death, and concomitantly decreased the DNA binding and transcriptional activity of NF-κB in cultured astrocytes. H2O2-induced astrocyte death was markedly increased by the co-treatment with pyrrolidinedithiocarbamate, an NF-κB inhibitor. Moreover, the elevation of constitutive NF-κB activity by overexpressing p65 NF-κB subunit attenuated H2O2 toxicity, whereas NF-κB inhibition by overexpressing IκB potentiated the toxicity. NF-κB activity and H2O2 cytotoxicity was further found to be dependent on cell density. Compared with astrocytes in low cell density, those in high cell density exhibited a higher constitutive NF-κB activity and a stronger resistance to H2O2 cytotoxicity. These results indicate that the constitutive activity of NF-κB in astrocytes is required for their survival under oxidative stress such as H2O2.
| Abbreviations | ||
| NF-κ_it;B | = | nuclear factor-kappaB |
| H2O2 | = | hydrogen peroxide |
| LDH | = | lactate dehydrogenase |
| PDTC | = | pyrrolidine dithiocarbamate |
| Iκ_it;B | = | inhibitory kappa B |
| Abbreviations | ||
| NF-κ_it;B | = | nuclear factor-kappaB |
| H2O2 | = | hydrogen peroxide |
| LDH | = | lactate dehydrogenase |
| PDTC | = | pyrrolidine dithiocarbamate |
| Iκ_it;B | = | inhibitory kappa B |