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Original

Docosahexaenoic acid supplementation induces dose and time dependent oxidative changes in C6 glioma cells

, , , , , , & show all
Pages 748-756 | Received 06 Oct 2006, Published online: 07 Jul 2009
 

Abstract

In view of the promising use of n-3 polyunsaturated fatty acids (PUFAs) in the prevention and treatment of neurological diseases, it is necessary to ascertain the lack of detrimental oxidative effects. We evaluated short- and long-term effects of 25, 50 and 75 μM docosahexaenoic acid (DHA) supplementation on the oxidative status of C6 glial cells. DHA was incorporated into cells dose and time dependently without any cytotoxic effect. Reactive oxygen species (ROS) level was related to DHA dose and supplementation time. At the lowest dose no significant increase in ROS values was observed at hour 24. Low doses of DHA strengthened the cellular antioxidant defence system as highlighted by a raise in both GPX and catalase activity, and the decreased levels of lipid peroxidation. This effect was pronounced at 24 h of supplementation, almost disappeared at hour 48, while after 72 h an opposite effect was observed: lipid peroxidation increased concomitantly with DHA doses. Therefore, the final effect of DHA on cellular redox status is dependent on dose and time supplementation.

Abbreviations
CAT=

catalase

DCF=

2′-7′-dichlorofluorescein

DHA=

docosahexaenoic acid

G6PDH=

glucose-6-phosphate dehydrogenase

GPX=

glutathione peroxidase

GSH=

reduced glutathione

LPO=

lipid peroxides

MTT=

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PLA2=

phospholipase A2

PUFA=

polyunsaturated fatty acids

ROS=

reactive oxygen species

SOD=

superoxide dismutase

TBARs=

thiobarbituric acid-reactive substances

Abbreviations
CAT=

catalase

DCF=

2′-7′-dichlorofluorescein

DHA=

docosahexaenoic acid

G6PDH=

glucose-6-phosphate dehydrogenase

GPX=

glutathione peroxidase

GSH=

reduced glutathione

LPO=

lipid peroxides

MTT=

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PLA2=

phospholipase A2

PUFA=

polyunsaturated fatty acids

ROS=

reactive oxygen species

SOD=

superoxide dismutase

TBARs=

thiobarbituric acid-reactive substances

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