![Sample our Food Science & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5936/TOC-Subject-Sample-2021-Food-Science-Technology.jpg"})
Abstract
Increased evidence from animal and in vitro cellular research indicates that the metabolism of eicosapentaenoic acid (EPA) can inhibit carcinogenesis in many cancers. Free radical-mediated peroxidation is one of many possible mechanisms to which EPA's anti-cancer activity has been attributed. However, no direct evidence has been obtained for the formation of any EPA-derived radicals. In this study, a combination of LC/ESR and LC/MS was used with α-[4-pyridyl 1-oxide]-N-tert-butyl nitrone to identify the carbon-centred radicals that are formed in lipoxygenase-catalysed EPA peroxidation. Of the numerous EPA-derived radicals observed, the major products were those stemming from β-scission of 5-, 15- and 18-EPA-alkoxyl radicals. By means of an internal standard in LC/MS, this study also quantified each radical adduct in all its redox forms, including an ESR-active form and two ESR-silent forms. The comprehensive profile of EPA's radical formation provides a starting point for ongoing research in defining the biological effects of radicals generated from EPA peroxidation.
| Abbreviations | ||
| ACN | = | acetonitrile |
| EIC | = | extracted ion current chromatogram |
| EPA | = | eicosapentaenoic acid |
| ESR | = | electron spin resonance |
| HOAc | = | glacial acetic acid |
| HPLC/LC | = | high performance liquid chromatography |
| IS | = | internal standard |
| LOX | = | lipoxygenase |
| MRM | = | multiple reaction monitoring |
| MS/MS | = | mass spectrometry |
| POBN | = | α-[4-pyridyl 1-oxide]-N-tert-butyl nitrone |
| PUFAs | = | polyunsaturated fatty acids |
| TIC | = | total ion current chromatogram |
| tR | = | retention time |
| Abbreviations | ||
| ACN | = | acetonitrile |
| EIC | = | extracted ion current chromatogram |
| EPA | = | eicosapentaenoic acid |
| ESR | = | electron spin resonance |
| HOAc | = | glacial acetic acid |
| HPLC/LC | = | high performance liquid chromatography |
| IS | = | internal standard |
| LOX | = | lipoxygenase |
| MRM | = | multiple reaction monitoring |
| MS/MS | = | mass spectrometry |
| POBN | = | α-[4-pyridyl 1-oxide]-N-tert-butyl nitrone |
| PUFAs | = | polyunsaturated fatty acids |
| TIC | = | total ion current chromatogram |
| tR | = | retention time |