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Abstract
Mitochondria are one of the major sources of reactive oxygen species (ROS) in mammalian cells. The generation of ROS underlies many physiological and pathophysiological processes that occur within cellular systems. Superoxide () is the proximal ROS generated during electron ‘leakage’ from the mitochondrial electron transport chain (mETC) and is known to be released at mitochondrial complex I and complex III. Monitoring mitochondrial
production directly and in real-time offers the potential to improve understanding of the complex mechanisms involved during mitochondrial
generation. This study reports the novel application of a cytochrome c functionalized amperometric sensor for monitoring
generation in isolated mitochondrial fractions. The non-invasive sensor system described allowed a comparison of
production following specific inhibition of complex I and complex III of the mETC to be made directly and in real-time.
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| mETC | = | mitochondrial electron transport chain |
| SOD | = | superoxide dismutase |
| XOD | = | xanthine oxidase |
| DTSSP | = | 3,3'-dithiobis(sulphosuccinimidylpropionate) |
| EPR | = | electron paramagnetic resonance |
| Abbreviations | ||
| ROS | = | reactive oxygen species |
| mETC | = | mitochondrial electron transport chain |
| SOD | = | superoxide dismutase |
| XOD | = | xanthine oxidase |
| DTSSP | = | 3,3'-dithiobis(sulphosuccinimidylpropionate) |
| EPR | = | electron paramagnetic resonance |