A Selective Separation Method for Slower‐Growing Bacteria from Marine Soils

Abstract

Egg lecithin greatly reduced the antibacterial activities of chlorhexidine gluconate (CHG). By using this antagonistic action, slower‐growing bacteria, for which a longer incubation time was needed for its growth, were isolated from marine soils. The growth of relatively faster‐growing bacteria, which formed visible colonies during a 3 day incubating period, was significantly inhibited by treatment with 8 µg of CHG per mL for 10 min, whereas the addition of egg lecithin to a final concentration of 2%, after the treatment, had no significant effect on the viability of slower‐growing bacteria.

Keywords:

• Environmental bacteria
• Isolation
• Chlorhexidine gluconate
• Lecithin