DIRECT ELECTROCHEMICAL DETERMINATION OF ADENINE USING HPMαFP-MODIFIED GLASSY CARBON ELECTRODE IN COMMERCIAL PHARMACEUTICAL PRODUCTS

Abstract

The electrochemistry properties of adenine on the 1-phenyl-3-methyl-4-(2-furoyl)-5-pyrazolone (HPMαFP)-modified glassy carbon electrode were investigated. The modified electrode showed an excellent electrocatalytic effect on the oxidation of adenine and displayed a shift of the oxidation potential in the negative direction with significant increase in the peak current. Under the optimum conditions, a linear calibration curve was obtained within the concentration range 5 × 10⁻⁷ to 1 × 10⁻⁴ M in 0.10 M phosphate buffer solution. The detection limit was 1.25 × 10⁻⁷ M. This developed method had been applied to the direct determination of adenine in tablet samples with satisfactory results.

Keywords:

• acylpyrazolone
• adenine
• glassy carbon electrode
• modified electrode

ACKNOWLEDGMENTS

This work was supported by grants from the Nature Science Foundation of Heilongjiang Province, P. R. China (No. B201004) and the Scientific and Technical Development Foundation of Harbin Normal University (No. 08XYG-12).