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Instrumentation Science & Technology Volume 45, 2017 - Issue 3
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Original Articles

Spectrofluorometric determination of ascorbic acid using thiamine and potassium ferricyanide

Ruilin DuanKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Junze JiangKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Shaopu LiuKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Jidong YangCollege of Chemical and Environmental Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing, China
,
Jinghui ZhuKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Man QiaoKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
,
Jingjing YanKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China
&
Xiaoli HuKey Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, ChinaCorrespondence[email protected]
 show all
Pages 312-323 | Published online: 02 Nov 2016
 
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ABSTRACT

A simple and sensitive spectrofluorometry method for the determination of ascorbic acid was reported. In alkaline solution, the nonfluorescent thiamine was oxidized by potassium ferricyanide and formed the fluorescent thiochrome with an excitation maximum at 367 nm and an emission peak at 441 nm. However, the fluorescence intensity gradually decreased in the presence of ascorbic acid. The decreased fluorescence was linearly dependent under the optimum conditions with the concentration of ascorbic acid from 0.086–1.5 µmol L−1 with a correlation coefficient of 0.9996. The detection limit of 0.026 µmol L−1 was lower than many other methods. Additionally, the mechanism underlying the enhancement and quenching of this method was discussed. The protocol was used to determine ascorbic acid in tablets and juice with satisfactory results.

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