Comparison of Chiral Separations in Capillary Zone Electrophoresis with Other Methods∗

∗ Presented in part at the Thirty-Second Annual Eastern Analytical Symposium, Somerset, NJ, November 1993, Paper #13.

Abstract

Capillary zone electrophoresis (CZE) is an efficient microseparation technique that can be used for the separation of small as well as large biomolecules. The use of micelles in the buffer makes CZE an acceptable technique for the resolution of charged and neutral molecules. The separation of chiral compounds is an important aspect of the pharmaceutical industry, synthetic organic chemistry and other areas. Optical isomers can be separated from each other using high pressure liquid chromatography (HPLC) and capillary gas chromatography (CGC), however, each of these techniques has its limitations. CGC and HPLC require a chiral column, which is costly, CZE does not. CZE has the highest efficiency and chiral separations are achieved by adding a modifier to the buffer that facilitates the separation of optical isomers.

In this review the advantages and disadvantages of HPLC, CGC and CZE for chiral separations will be discussed.

∗ Presented in part at the Thirty-Second Annual Eastern Analytical Symposium, Somerset, NJ, November 1993, Paper #13.

Notes

∗ Presented in part at the Thirty-Second Annual Eastern Analytical Symposium, Somerset, NJ, November 1993, Paper #13.