Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system–Epic^® system

Abstract

The Epic^® system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.

Keywords::

• Label free
• phosphor-specific
• Epic^®
• 14-3-3
• kinase

Acknowledgments

We thank members of the Li laboratory for valuable comments on this manuscript. This work is supported by a grant from the National Institutes of Health (to M.L. U54 MH084691, RO1 GM078579), and supported in part by Corning Inc.

Declaration of interest: The authors report no conflicts of interest.