The role of α₁-adrenergic receptors in regulating metabolism: increased glucose tolerance, leptin secretion and lipid oxidation

Abstract

The role of α₁-adrenergic receptors (α₁-ARs) and their subtypes in metabolism is not well known. Most previous studies were performed before the advent of transgenic mouse models and utilized transformed cell lines and poorly selective antagonists. We have now studied the metabolic regulation of the α_1A- and α_1B-AR subtypes in vivo using knock-out (KO) and transgenic mice that express a constitutively active mutant (CAM) form of the receptor, assessing subtype-selective functions. CAM mice increased glucose tolerance while KO mice display impaired glucose tolerance. CAM mice increased while KO decreased glucose uptake into white fat tissue and skeletal muscle with the CAM α_1A-AR showing selective glucose uptake into the heart. Using indirect calorimetry, both CAM mice demonstrated increased whole body fatty acid oxidation, while KO mice preferentially oxidized carbohydrate. CAM α_1A-AR mice displayed significantly decreased fasting plasma triglycerides and glucose levels while α_1A-AR KO displayed increased levels of triglycerides and glucose. Both CAM mice displayed increased plasma levels of leptin while KO mice decreased leptin levels. Most metabolic effects were more efficacious with the α_1A-AR subtype. Our results suggest that stimulation of α₁-ARs results in a favorable metabolic profile of increased glucose tolerance, cardiac glucose uptake, leptin secretion and increased whole body lipid metabolism that may contribute to its previously recognized cardioprotective and neuroprotective benefits.

Keywords:

• Adrenergic receptor
• fatty acid oxidation
• leptin
• metabolism

Acknowledgments

The authors wish to thank Satish Kalhan, MD, of the Cleveland Clinic for his consulting and insight into these metabolic studies.

Disclosure statement

The authors report no conflicts of interest.

Funding

This work was supported by a grant from The National Institutes of Health [1R03AG049394], an American Heart Association Grant in Aid (Great Rivers Affiliate) to DMP, and the Case Western Reserve University Mouse Metabolic Phenotyping Center (MMPC) grant [U24 DK76174].