Inhibition of TGF-β1 on Gli2 expression was promoted by TNF-α in primary leukemia cells

Abstract

Purpose

Hedgehog (Hh) signaling pathway regulates a variety of tumors-related diseases including leukemia. Whether inhibition of TGF-β1 on Gli2 expression is promoted by TNF-α in primary leukemia cells remains to be determined.

Methods

Primary leukemia cells were treated with TGF-β1, TNF-α or SIS3 at different concentrations. Gli2 expression was detected by quantitative real-time PCR and western blot analyses.

Results

We found that TGF-β significantly decreased Gli2 expression, and co-treatment with TNF-αfurther decreased Gli2 expression in primary leukemia cells. TNF-α can increased TGF-βRI and TGF-βRII protein expression in primary leukemia cells, while SIS3 inhibited the effect of TGF-β.

Conclusion

Our results suggest that Gli2 expression in primary leukemia cells is induced by TGF-β in a Smad3-dependent manner, and independent of Hh receptor signaling.

Keywords:

• Primary leukemia cells
• TGF-β1
• TNF-α
• Gli2

Ethic approval

This study was approved by the ethical commitee of the First Affiliated Hospital of Jinzhou Medical University. And Informed consents were obtained from the patients

Disclosure statement

I, Zhe Li, certify that this research has not contributed to any other journals. On behalf of myself and my coauthors, we have no relevant financial interest in this manuscript.