Adrenergic receptor behaviors of mesenchymal stem cells obtained from different tissue sources and the effect of the receptor blockade on differentiation

Abstract

In this study, it was aimed to analyze behavioral changes of adrenergic receptors (ARs) in first three passages and osteogenic/adipogenic differentiation of mesenchymal stem cells (MSCs) derived from placenta fetal membrane (FM) and bone marrow (BM). It was also aimed to evaluate effects of receptor blockade on differentiation. We obtained first three passages of MSCs from placenta and BM samples. For cell identification, the cells were analyzed by flow cytometry using CD34, CD45 and CD3, CD105 antibodies in each passage. The effects of propranolol and phenoxybenzamine at incremental doses were analyzed by MTT. In addition, cell cultures were separately maintained with the blockers or without after second passage. After each passage and differentiation, α1A, α1B, α2A, α2B, β1, β2, β3 AR-mRNA expressions analyzed by RT-qPCR technique. BMP6 and PPARG mRNA expressions only after differentiation and passage 3 were analyzed. A microscopic examination was also performed. Our results showed that AR expression behaviors were different in MSCs obtained from different tissue sources. In particular, α_1A-AR and α_2A-AR were expressed with considerably high coefficients in differentiation under blocker effect in BM-derived MSCs. No such coefficients were observed in any group of placental MSCs. In addition, it was found that the blockers stimulated adipogenesis in BM-derived MSCs during osteogenic differentiation. MSCs exhibit protein expressions that vary according to source of tissue and differentiation. Given that MSCs from different sources are used for repair and modulation, our study makes implications of this variable expression intriguing in the clinical practice.

Keywords:

• Mesenchymal stem cells
• adrenergic receptors
• differentiation

Acknowledgements

The results presented in this paper were all of a PhD thesis. The authors would like to thank Dilara Nemutlu Samur and the staff of Başkent University Adana Adult Bone Marrow Transplantation Center for their contribution to the study and the preparation of this manuscript. This study was approved by The Ethics Committee on Non-interventional Clinical Research of Çukurova University (decision number: 2013-21-6).

Author contributors

EM designed the study, conducted all its phases and wrote the article, AAY contributed to the conduct and analysis of cell culture experiments. IK as the thesis second supervisor, FA as the thesis supervisor, they checked every phase of the work and intervened where necessary. IK and FA also revised the article it for critically important content.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was supported by the Çukurova University Research Fund [Project No.: TF2013D1].