Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Abstract

Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI‐TOF‐MS and ESI‐MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.

*Equal contribution

Keywords:

• Ionizing radiation
• Oxidative stress
• ROS
• Proteomics

Acknowledgments

This work was supported by NIH CA109480, CA111846, U.S. Army PC050127, Korea Research Foundation Grant KRF-2004-005-C00112, and University of Incheon.

Notes

*Equal contribution