Critical factors affecting ethanol production by immobilized Pichia stipitis using corn cob hemicellulosic hydrolysate

ABSTRACT

Fermentation of xylose from hydrolysate of acid-treated corn cob by Pichia stipitis is inhibited by acetic acid and lignin derivatives. In the present study, we have designed and implemented an immobilized cell culture for xylose to ethanol conversion from acid-treated corn cob hydrolysate without the removal of fermentation inhibitors. In this study, cultivations of suspended and immobilized Pichia were compared in terms of ethanol yield and productivity to investigate whether the cell immobilization could improve resistance to inhibitors. Cell immobilization clearly favored the fermentative metabolism in nondetoxified corn cob hydrolysate leading to an improvement of twofold ethanol productivity as compared to that achieved with suspension culture. Calcium alginate as an immobilization matrix was selected to immobilize Pichia cells. Concentrations of sodium alginate, calcium chloride, and fermentor agitation speed were optimized for ethanol production using statistical method. Statistical analysis showed that agitation speed had maximum influence on ethanol production by immobilized Pichia cells. In comparison to suspension culture, immobilization had a positive impact on the fermentative metabolism of Pichia, improving the ethanol yield from 0.40 to 0.43 g/g and productivity from 0.31 to 0.51 g/L/h for acid-treated corn cob hydrolysate.

KEYWORDS:

• Agitation
• corn cob hydrolysate
• immobilization
• Pichia stipites
• xylose

Abbreviations:

• CC: calcium chloride
• DO: dissolved oxygen
• E: ethanol (g/L)
• Q_p: ethanol volumetric productivity
• SA: sodium alginate
• Y_p/s: ethanol conversion yield
• hr: time (hr)

Acknowledgments

The authors gratefully acknowledge Praj Industries Ltd. Pune for financial support to conduct the research work. The authors are also thankful to analytical team of Praj Matrix-R&D Center for providing assistance with analytical measurements.