Biosynthesis of butyric acid by Clostridium tyrobutyricum

ABSTRACT

Butyric acid (C₃H₇COOH) is an important chemical that is widely used in foodstuffs along with in the chemical and pharmaceutical industries. The bioproduction of butyric acid through large-scale fermentation has the potential to be more economical and efficient than petrochemical synthesis. In this paper, the metabolic pathways involved in the production of butyric acid from Clostridium tyrobutyricum using hexose and pentose as substrates are investigated, and approaches to enhance butyric acid production through genetic modification are discussed. Finally, bioreactor modifications (including fibrous bed bioreactor, inner disk-shaped matrix bioreactor, fibrous matrix packed in porous levitated sphere carriers), low-cost feedstocks, and special treatments (including continuous fermentation with cell recycling, extractive fermentation with solvent, using different artificial electron carriers) intended to improve the feasibility of commercial butyric acid bioproduction are summarized.

KEYWORDS:

• Biomass feedstock
• butyric acid
• Clostridium tyrobutyricum
• fibrous bed bioreactor
• genetic modification

Additional information

Funding

The research was supported by grants from the Natural Science Foundation of China (NSFC) (No.21406204), and the Zhejiang Provincial Natural Science Foundation (No.LQ12B06005), PR China.