Production of β-glucanase and protease from Bacillus velezensis strain isolated from the manure of piglets

Abstract

In this study, a strain producing β-glucanase and protease, identified as Bacillus velezensis Y1, was isolated from the manure of piglet. We attempted to produce β-glucanase and protease after optimization of various process parameters with the solid state fermentation. The effects of each factor on producing β-glucanase and protease were as follows: temperature > time > pH > loaded liquid volume. The properties of the β-glucanase showed that the most suitable reaction temperature was 65 °C and pH was 6.0. However for protease optimum reaction temperature was 50 °C, and pH was 6.0. The amplified PCR fragments of β-glucanase and protease were 1434 bp containing an open reading frame of 1413 bp encoding a protein with 444 amino acids and 1752 bp containing an open reading frame of 1521 bp encoding a protein with 506 amino acids, respectively. So, the study demonstrated a viable approach of using newly identified B. velezensis Y1 strain for the maximum yield of two industrially important enzymes.

Graphical Abstract

Keywords:

• Bacillus velezensis
• β-glucanase
• enzyme properties
• fermentation conditions
• protease

Disclosure statement

No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the [Major Science and Technology Program of Anhui Province 1] under grant [201903a06020020]; [Fund of College of Natural Science from Anhui Province 2] under grant [KJ2018A0141]; Anhui Industry and Technology System of Poultry Science 3] under grant [AHCYTX-10]; [Fund of State Key Laboratory of Animal Nutrition 4] under grant [2004DA125184F1725]; and [Natural Science Foundation of China 5] under grant [31571506].