Production of cellulase by Novosphingobium sp. Cm1 and its potential application in lignocellulosic waste hydrolysis

Abstract

Management of lignocellulosic wastes in and around the municipality area requires special consideration. Continuous deposition of these wastes to the nearby areas led to gradual deterioration of the environment. The objective of this study was to produce cellulase from the bacteria isolated from the unexplored rainforest of NE-India for lignocellulosic waste hydrolysis. Based on carboxymethyl cellulose utilization and the congo red test, Novosphingobium sp. Cm1 was found to be the most promising strain out of 114 bacterial isolates and the strain was selected for further study. The optimization of the fermentative conditions for maximum enzyme activity was carried out using one factor-at-a-time strategy and the optimum pH, temperature and incubation time was recorded as pH 5, 37 °C and 96 h respectively. The maximum β–1,4–endoglucanase activity was observed with 1.5% CMC (5.1 ± 0.05 U/mL) and 0.25% yeast extract (7.6 ± 0.72 U/mL). The bacterial waste hydrolysis ability was investigated using three wastes where vegetable waste showed maximum activity of 3.4 ± 0.48 U/mL. Bacterial interaction and waste utilization were verified using Scanning Electron Microscope and Fourier-Transform infrared spectroscopy analysis. The present study confirmed the promising ability of Novosphingobium sp. to waste hydrolysis. Further investigations may lead to new possibilities for low-cost enzyme production that will help to meet the rising cellulase demand.

Keywords:

• Cellulose
• endoglucanase
• optimization
• saccharification
• vegetable waste

Acknowledgments

The authors are thankful to the Director CSIR-NEIST, Jorhat for proving necessary facilities to carry out the research work.

Disclosure statement

The authors confirm that there is no conflict of interest.

Additional information

Funding

The first author is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi for awarding fellowship as CSIR-Senior Research Fellow.