Abstract
This work deals with the optimization of an extracellular phospholipase C production by Bacillus cereus (PLCBc) using Response Surface Methodology (RMS) and Box-Behnken design. In fact, after optimization, a maximum phospholipase activity (51 U/ml) was obtained after 6 h of cultivation on tryptone (10 g/L), yeast extract (10 g/L), NaCl (8.125 g/L), pH 7.5 with initial OD (0.15). The PLCBc activity, esteemed by the model (51 U) was very approximate to activity gutted experimentally (50 U). The PLCBc can be considered as thermoactive phospholipase since it showed a maximal activity of 50 U/mL at 60 °C using egg yolk or egg phosphatidylcholine (PC) as substrate. In addition, the enzyme was active at pH 7 and is stable after incubation at 55 °C for 30 min. The application of B. cereus phospholipase C in soybean oil degumming was investigated. Our results showed that when using enzymatic degumming, the residual phosphorus decrease more than with water degumming, indeed, it passes from 718 ppm in soybean crude oil to 100 ppm and 52 ppm by degumming using water and enzymatic process, respectively. The diacylgycerol (DAG) yield showed an increase of 1.2% with enzymatic degumming compared to soybean crude oil. This makes our enzyme a potential candidate for food industrial applications such as enzymatic degumming of vegetable oils.
Acknowledgment
We are grateful to Dr A. Verwey (Agrolab Roterdam, Netherlands) for DAG analysis.
Author contributions
Ines Abdelkader: Data curation, Formal analysis, Investigation, Methodology, Writing-Original Draft. Sameh Ben Mabrouk and Bilel Hadrich: Inversigation, Methodology, Writing – Review & Editing. Ahmed Fendri: Supervision, Writing – Review & Editing. Adel Sayari: Conceptualization, Writing – Review & Editing, Supervision, Project administration.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
All data generated during this study are included in the submitted manuscript.