Large Scale Extraction of α-Lactalbumin and β-Lactoglobulin from Bovine Whey by Precipitation with Polyethylene Glycol and Partitioning in Aqueous Two-Phase Systems

Abstract

The milk proteins α-lactalbumin and β-lactoglobulin have been isolated from bovine whey by fractional precipitation with polyethylene glycol (PEG) and hydrophobic partitioning in aqueous PEG-hydroxypropylstarch two-phase systems using PEG-bound palmitate as hydrophobic ligand. The possible use of this combination for large scale purification of these whey proteins is discussed.