Matrix Solid‐Phase Dispersion Combined with Thin‐Layer Chromatography–Direct Bioautography for Determination of Enrofloxacin and Ciprofloxacin Residues in Milk

Abstract

Enrofloxacin is a common veterinary antibiotic, which can be found as a residue in milk, together with its main metabolite, ciprofloxacin. Chromosorb WAW was used as a sorbent for matrix solid‐phase dispersion of milk samples spiked with these antibiotics. This pre‐separation method was combined with thin‐layer chromatography‐direct bioautography to obtain semi‐quantitative results. Various modes of the procedure were tested and the one giving the best recovery of the antibiotics from milk was chosen.

Keywords:

• Enrofloxacin
• Ciprofloxacin
• Thin‐layer chromatography–direct bioautography
• Matrix solid‐phase dispersion