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Abstract
Fifteen selected essential oil components and aroma substances were investigated using adsorption thin‐layer chromatography on silica gel 60 (E. Merck, #1.05744) and aluminum oxide (E. Merck, #1.05550) plates and the mobile phases benzene–chloroform, benzene–acetone, carbon tetrachloride–acetone, chloroform–acetone, and chlorobenzene–acetone in different volume compositions. The separations were evaluated on the basis of retention parameter RF and separation parameters values ΔRF, α, and RF α. None of the applied chromatographic conditions allowed for the complete separation of all the compounds examined. Aluminium oxide plates and carbon tetrachloride–acetone in volume composition 98:2 were optimal for the separation of the investigated alcohols: (+) borneol (B), geraniol (GR), menthol (M), and linalool (L). Aluminium oxide and carbon tetrachloride–acetone mobile phases in a volume composition of 85:15 were optimal for the separation of the investigated phenols: vanillin (V), guaiacol (G), eugenol (E), and thymol (T). Aluminium oxide and a chlorobenzene–acetone mobile phase in a volume composition of 95:5 was optimal for the separation of coumarin (C), cineole (CL), (R)‐(−) carvone (CR), cinnamic aldehyde (CA), camphor (CP), (1R)‐(−) fenchone (F), and trans anethole (A).