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Original Articles

Interaction of Hydroxypropyl‐β‐Cyclodextrin with Peptides, Studied by Reversed‐Phase Thin‐Layer Chromatography

, , , , , & show all
Pages 2619-2632 | Received 13 Dec 2004, Accepted 17 Feb 2005, Published online: 06 Feb 2007
 

Abstract

The effect of various concentrations of hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) on the reversed‐phase thin‐layer chromatographic mobility of some low molecular mass peptides was measured in distilled water and in 0.05 M of LiCl, NaCl, KCl, RbCl, and CsCl solutions, and the relative strength of HP‐β‐CD–peptide interaction has been calculated from the dependence of retention on the concentration of HP‐β‐CD in the mobile phase. Lipophilicity of the peptides decreased regularly with increasing concentration of HP‐β‐CD in the mobile phase, proving the interaction (probably the formation of inclusion complexes) between peptides and HP‐β‐CD. Principal component analysis indicated that each salt influenced the interaction differently. Peptides formed clusters on the two‐dimensional nonlinear map according to the character of the amino acid monomer and not according to the number of amino acid units, suggesting that only one terminal amino acid is included in the cavity of HP‐β‐CD.

Acknowledgment

This work was supported by the cooperation between the Institute of Physiology of the Academy of Sciences of the Czeh Republic and the Research Institute of Materials and Environmental Chemistry, Chemical Research Center, Hungarian Academy of Sciences.

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