Abstract
RP‐HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis™ C18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25°C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4–CH3OH (95∶5, pH 2.7), a flow rate of 0.8 mL/min and 10 µL sample size. The detection limits ranged 3.2–619 ng/mL, the coefficients of variation ranged 1.3–4.6%, and the recoveries ranged 95.6–106.3% for soil solution with standard addition on the optimal conditions proposed.
Acknowledgments
The authors are grateful to the National Basic Research Program of China from MOST (2005CB121107), the National Science foundation of China (40371067), and the Key Project of Knowledge Innovation System of Chinese Academy of Sciences (KZCX3‐SW‐417).