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Abstract
A simple and selective high performance liquid chromatographic method has been developed for the determination of total iron in water samples. 2‐(8‐quinolylazo)‐4,5‐diphenylimidazole (QAI) was used as a precolumn chelating reagent for iron(II). The iron(II)‐QAI chelate was separated on a Cosmosil 5C8‐MS column with an aqueous acetonitrile mobile phase containing tetrabutylammonium bromide and spectrophotometrically detected at 710 nm. Iron(III) was determined as the iron(II) chelate with QAI after it was reduced to iron(II) using ascorbic acid. Detection limit, defined as three times of standard deviation of a blank signal, was 18 pg of iron(II) in 100 µL injection. Total iron in environmental water samples was selectively determined without any pretreatment.