Influence of Zinc and Nickel Sulfates as Mobile Phase Additives on the Retention, Efficiency, and Selectivity of Organic Hydrocarbons with Polar Groups in RP‐HPLC

Abstract

Inorganic eluent additives affect the retention and selectivity of chosen hydrocarbons with polar proton‐acceptor groups in reversed phase HPLC. Following that, nickel and zinc sulfates have been used in organic‐aqueous mobile phase at the concentration ranging from 0.001 to 0.01 M. The influence of salts is attributed to the desolvation of the analytes mainly due to the sulfate anions in most cases, or selective complexation with particular entities by metal cathions.

The effect of salts concentrations on efficiency on a RP‐18‐bonded silica column has also been studied.

All investigated compounds displayed significant changes in efficiency with the increase of salt concentration.

Keywords:

• Antychaotropic mobile phase additives
• RP‐HPLC
• Organic hydrocarbons with polar groups
• Retention behavior
• Separation selectivity
• Efficiency of chromatographic systems