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Original Articles

Preparative Isolation of Mangiferin from Anemarrhena asphodeloides Rhizomes by Centrifugal Partition Chromatography

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Pages 869-875 | Received 15 Nov 2005, Accepted 10 Dec 2005, Published online: 06 Feb 2007
 

Abstract

Mangiferin was separated from rhizomes of Anemarrhena asphodeloides extract using centrifugal partition chromatography (CPC) with a two‐phase solvent system composed of ethyl acetate‐isopropanol‐water (3∶2∶5, v/v). Mangiferin (22.5 mg) was successfully isolated from methanolic extracts (957 mg) in only one step, and the purity of isolated compound was determined to be over 95% by HPLC analysis. The structure of mangiferin was identified by 1H, 13C NMR and ESI‐MS spectral data analysis.

Acknowledgment

This work was supported by the Korea Research Foundation Grant (KRF‐2003‐015‐E00217).

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