Determination of Dissociation Constants of Resverastrol and Polydatin by Capillary Zone Electrophoresis

Abstract

A method to determine the dissociation constants of resverastrol and polydatin by capillary zone electrophoresis was developed. First, resverastrol and polydatin have to be baseline separated to measure their effective electrophoretic mobilities accurately. Second, the first order dissociation constants of resverastrol and polydatin, which are 9.49 and 9.40, can be obtained from the non‐linear regression between their effective electrophoretic mobilities and the H⁺ activities of the running buffer. The correlation coefficients of the non‐linear regression are 0.9956 and 0.9982, respectively. The reliability of the method is validated by the result of the hydroquinone of which the first order dissociation constant is known.

Keywords:

• Resverastrol
• Polydatin
• Dissociation constant
• Non‐linear regression
• Capillary zone electrophoresis