Determination of the Stabilities of New Quinazoline Derivatives by HPLC

Abstract

The quinazoline derivatives reported in this work belong to a new group of compounds with a type of chemical bonding that has potential anticancer features. A biochemical study determining anticancer activity of quinazoline derivatives and N³‐quinazoline oxide will be conducted in the medium of 0.2% DMSO. The HPLC method was used to determine stabilities of these compounds in a biochemical study environment. Optimisation of the chromatographic system and validation of the established analytical method was performed. Analysis of the stabilities of the derivatives examined was performed in the reversed phase RP‐18 system, in which pure acetonitrile was used as an eluent for the analysis of compounds 1 and 3, and a mixture of acetonitrile/methanol in the ratio of 95/5% for compounds 2 and 4. The HPLC analysis revealed that the compounds studied are homogeneous in the 0.2% DMSO medium, up to 96 hours.

Keywords:

• HPLC
• RP‐18
• Quinazoline
• Quinazoline N³‐oxide

Acknowledgment

The HPLC analytical research was supported by the Medical University of Łódź, Poland (Grant No. 507 13 028).