High Temperature High Performance Liquid Chromatography of Substituted Anilines using a C₁₈ Hybrid Stationary Phase

Abstract

Separation of twelve substituted anilines in less than ten minutes is reported. A C₁₈ hybrid stationary phase and a mobile phase consisting of pure water was used for separation. To separate the analytes a temperature program between 160°C and 200°C with 5°C/min was used. Under isothermal conditions van't Hoff plots were constructed between 150°C and 200°C. All the plots were straight lines indicating only one type of interaction between the analytes and the stationary phase. The compounds were detected using either UV or simultaneous UV and FID detection.

Keywords:

• Hybrid stationary phase
• van't Hoff plots
• UV detection
• FID detection